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Abnova
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Meso Scale Diagnostics LLC
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BASF
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Bio-Techne corporation
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Bio-Techne corporation
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Bio-Techne corporation
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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: A Human Anti-Insulin IgG Autoantibody Apparently Arises Through Clonal Selection from an Insulin-Specific “Germ-Line” Natural Antibody Template
doi:
Figure Lengend Snippet: Binding of the recombinant VHDJH13/VκJκ13 (A), VH13G12-DJH13/Vκ13K20-Jκ13 (B), Lys52/VκJκ13 (C), and Arg58/VκJκ13 (D) IgG1 Abs to human recombinant insulin (●), ssDNA (△), tetanus toxoid (■), human recombinant IL-1β (▲), E. coli β-galactosidase (◇), and BSA (□). The Ag-binding activity of each Ab to solid-phase Ag is expressed as optical absorbance at 492 nm.
Article Snippet: Eluates were brought to pH 7.5 by addition of neutralizing buffer (pH 9.0), dialyzed against PBS, and stored in aliquots at 4°C. mAb concentration and binding to human recombinant insulin (Eli Lilly Research Laboratories, Indianapolis, IN),
Techniques: Binding Assay, Recombinant, Activity Assay